Recombinant CD16/FCGR3 Monoclonal Antibody (AN300509P)
For research use only.
| Verified Samples |
Verified Samples in WB:?C6 Verified Samples in IP: RAW246.7 |
| Dilution | WB 1:500-1:2000, IP 0.5-2 μL/mg of lysate |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Mouse |
| Applications | WB, IP |
| Clonality | Monoclonal |
| Immunogen | Recombinant Mouse CD16/FCGR3 protein |
| Abbre | FCGR3 |
| Synonyms | CD-antigen, Fcgr, CD16, CD16 Protein, CD-antigen 16, Fc gamma Receptor III, FcRIII, IgG Fc receptor III, Low affinity immunoglobulin gamma Fc region receptor III, Mouse, Fcgr3 |
| Swissprot | |
| Calculated MW | 29 kDa |
| Observed MW |
38 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Clone No. | 9C1 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | Fc receptors bind the most common class of antibody, IgG, are called Fc gamma receptors (FcγR). FcγR is divided into three classes, Fc γ RI (CD64), Fc γ RII (CD32), and Fc γ RIII (CD16). CD16 protein is a multifunctional, low/intermediate affinity receptor, which belongs to the immunoglobulin superfamily. It is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. Mouse CD16 is encoded by a single gene, while, human CD16 is expressed as two distinct forms (CD16a/FcγRIIIa and CD16b/FcγRIIIb) encoded by two different highly homologous genes in a cell type-specific manner. CD16 is involved in phagocytosis, secretion of enzymes, inflammatory mediators, antibody-dependent cellular cytotoxicity (ADCC), and clearance of immune complexes. |
Other Clones
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Other Formats
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Unconjugated
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