Recombinant Cathepsin D/CTSD Monoclonal Antibody (AN300423P)
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For research use only.
| Verified Samples |
Verified Samples in WB:?MCF7, A431, HepG2, SKBR3, 293T, CTSD konckout 293T Verified Samples in IHC: Human lung cancer, Human cirrhosis |
| Dilution | WB 1:500-1:2000, IHC-P 1:100-1:500 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB, IHC-P |
| Clonality | Monoclonal |
| Immunogen | Recombinant Human Cathepsin D/CTSD protein |
| Abbre | CTSD |
| Synonyms | CLN, CLN10, CPSD, HEL-S-130P, Cathepsin D, CTSD |
| Swissprot | |
| Calculated MW | 45 kDa |
| Observed MW |
31 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Tissue Specificity | Expressed in the aorta extracellular space (at protein level) |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Neuroscience, Signal Transduction, Cancer, Cell Biology |
| Clone No. | 3G8 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | This gene encodes a member of the A1 family of peptidases. The encoded preproprotein is proteolytically processed to generate multiple protein products. These products include the cathepsin D light and heavy chains, which heterodimerize to form the mature enzyme. This enzyme exhibits pepsin-like activity and plays a role in protein turnover and in the proteolytic activation of hormones and growth factors. Mutations in this gene play a causal role in neuronal ceroid lipofuscinosis-10 and may be involved in the pathogenesis of several other diseases, including breast cancer and possibly Alzheimer's disease. |
Other Clones
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Other Formats
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Unconjugated
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