Recombinant BMAL1 Monoclonal Antibody (AN300681L)
For research use only.
| Verified Samples | Verified Samples in WB: Rat brain |
| Dilution | WB 1:2000-1:10000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human BMAL1 protein |
| Abbre | BMAL1 |
| Synonyms | JAP, MOP, MIC, Pgp, CDW, BMAL, PASD, CSPG, bHLHe, MDU, ARNTL, BMAL1, BMAL1c, JAP3, MOP3, PASD3, TIC, bHLHe5, aryl hydrocarbon receptor nuclear translocator like, IN, LHR, MC56, MDU2, MDU3, MIC4, Pgp1, CDW44, CSPG8, HCELL, HUTCH-I, ECMR-III, ARNT like protein 1 brain and muscle, Aryl hydrocarbon receptor nuclear translocator like protein 1, Aryl hydrocarbon receptor nuclear translocator-like protein 1, Basic helix loop helix PAS orphan MOP3, Basic helix loop helix PAS protein MOP3, Basic-helix-loop-helix-PAS protein MOP3, bHLH PAS protein JAP3, bHLH-PAS protein JAP3, BMAL 1, Brain and muscle ARNT like 1, Brain and muscle ARNT-like 1, CG8727 PA, Class E basic helix-loop-helix protein 5, cycle, JAP 3, Member of PAS protein 3, Member of PAS superfamily 3, MGC47515, MOP 3, PAS domain-containing protein 3, PASD 3 |
| Swissprot | |
| Calculated MW | 69 kDa |
| Observed MW |
78 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Nucleus |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Cardiovascular, Epigenetics and Nuclear Signaling, Neuroscience, Metabolism |
| Clone No. | 6A2 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | The protein encoded by this gene is a basic helix-loop-helix protein that forms a heterodimer with CLOCK. This heterodimer binds E-box enhancer elements upstream of Period (PER1, PER2, PER3) and Cryptochrome (CRY1, CRY2) genes and activates transcription of these genes. PER and CRY proteins heterodimerize and repress their own transcription by interacting in a feedback loop with CLOCK/ARNTL complexes. Defects in this gene have been linked to infertility, problems with gluconeogenesis and lipogenesis, and altered sleep patterns. Several transcript variants encoding different isoforms have been found for this gene. |
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