Recombinant BLyS/TNFSF13B/BAFF Monoclonal Antibody (AN300173P)
For research use only.
| Verified Samples |
Verified Samples in WB: HL-60, Jurkat, MOLT-4 Verified Samples in IP: Hela, Jurkat |
| Dilution | WB 1:500-1:2000, IP 1-4 μL/mg of lysate |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB, FCM, IP |
| Clonality | Rabbit Monoclonal |
| Immunogen | Recombinant Human BLyS / TNFSF13B / BAFF protein |
| Abbre | TNFSF13B |
| Synonyms | UNQ, PRO, ZTNF, TNF- and APOL-related leukocyte expressed ligand, TNFSF, TNFSF13B, BAFF, BLYS, CD257, DTL, TALL-1, TALL1, THANK, TNFSF20, TNLG7A, ZTNF4, Tumor necrosis factor ligand superfamily member 13B, B lymphocyte stimulator, B-cell-activating factor, Dendritic cell-derived TNF-like molecule, TNF- and APOL-related leukocyte expressed ligand 1, UNQ401, PRO738, BAFF, TNFSF13b, TALL-1, ApoL related ligand TALL 1, B cell Activating Factor, CD 257, CD257 antigen, Delta BAFF, Dendritic cell derived TNF like molecule, DTL precursor, soluble form, TALL 1, TN13B, TNF and APOL related leukocyte expressed ligand 1, TNF homolog that activates apoptosis, TNF homolog that activates apoptosis NKFB and JNK., Tumor necrosis factor (ligand) superfamily member 13b, Tumor necrosis factor ligand 7A, Tumor necrosis factor ligand superfamily member 20, Tumor necrosis factor like protein ZTNF4, Tumor necrosis factor superfamily member 13B |
| Swissprot | |
| Calculated MW | 31 kDa |
| Observed MW |
31 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cell membrane, Secreted. |
| Tissue Specificity | Peripheral blood Leukocytes, Spleen, Lymph node, Bone marrow. |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Cell Biology, Immunology, Cancer |
| Clone No. | 7B3 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | BAFF, a member of the TNF superfamily of proteins, is a homotrimeric transmembrane protein, which is cleaved to produce a soluble cytokine. BAFF may also further oligomerize into 60-mer structures. BAFF is expressed by monocytes, neutrophils, macrophages, dendritic cells, activated T cells, and epithelial cells. BAFF plays a key role in B cell development, survival, and activation. BAFF binds to three distinct receptors, BAFF-R, TACI, and BCMA. These receptors are differentially expressed during B cell development and among B cell subsets. While BAFF-R and BCMA bind to the homotrimeric form of BAFF, TACI only binds to membrane bound or higher order BAFF structures. The BAFF/ BAFF-R interaction activates both canonical and non-canonical NF-κB pathways, PI3K/Akt, and mTOR. Activation of the noncanonical NF-κB pathway via BAFF-R is negatively regulated by TRAF3. Research studies have shown that elevated levels of BAFF may exacerbate many autoimmune disorders, making it a potential therapeutic target. |
Other Clones
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Other Formats
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Unconjugated
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