Recombinant BAT3 Monoclonal Antibody (E-AB-81460)
For research use only.
| Verified Samples |
Verified Samples in WB: K562, Rat Rat Brain, C6, 3T3 Verified Samples in IF: MCF-7 |
| Dilution | WB 1:1000-1:2000, IF 1:20-1:100 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IF |
| Clonality | Rabbit Monoclonal |
| Immunogen | A synthetic peptide of human BAT3 |
| Abbre | BAT3 |
| Synonyms | BAG-6, BAG6, BAT3, D6S52E, G3 |
| Swissprot | |
| Calculated MW | 119 kDa |
| Observed MW |
150 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm>cytosol. Nucleus. The C-terminal fragment generated by caspase-3 is cytoplasmic. |
| Concentration | 300 μg/mL |
| Buffer | 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% stabilizer and 0.05% protective protein. |
| Purification Method | Affinity Purified |
| Research Areas | Cancer, Cell Biology |
| Clone No. | R04-1C1 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | This gene was first characterized as part of a cluster of genes located within the human major histocompatibility complex class III region.This gene encodes a nuclear protein that is cleaved by caspase 3 and is implicated in the control of apoptosis.In addition, the protein forms a complex with E1A binding protein p300 and is required for the acetylation of p53 in response to DNA damage.Multiple transcript variants encoding different isoforms have been found for this gene. |
Other Clones
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Other Formats
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Unconjugated
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