Recombinant ATG7 Monoclonal Antibody (AN300970L)
For research use only.
| Verified Samples | Verified Samples in WB: Hela |
| Dilution | WB 1:1000-1:5000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human ATG7 protein |
| Abbre | ATG7 |
| Synonyms | GSA, ATG, Apg7/Atg, ATG7, APG7-LIKE, APG7L, GSA7, Apg7/Atg7, 1810013K23Rik, Apg 7, APG7, APG7 autophagy 7 like, APG7 autophagy 7-like (S. cerevisiae), APG7 like, ATG 7, ATG12-activating enzyme E1 ATG7, ATG7 autophagy related 7 homolog, ATG7 autophagy related 7 homolog (S. cerevisiae), Atg7l, Autophagy 7, Autophagy related protein 7, Autophagy-related 7 (yeast), Autophagy-related protein 7, DKFZp434N0735, GSA 7, hAGP7, homolog of, S. cerevisiae, Ubiquitin activating enzyme E1 like protein, Ubiquitin-activating enzyme E1-like protein, Ubiquitin-like modifier-activating enzyme ATG7, APG7 |
| Swissprot | |
| Calculated MW | 78 kDa |
| Observed MW |
78 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Cell Biology, Signal Transduction, Cardiovascular, Cancer, Metabolism |
| Clone No. | 10A4 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | This gene encodes an E1-like activating enzyme that is essential for autophagy and cytoplasmic to vacuole transport. The encoded protein is also thought to modulate p53-dependent cell cycle pathways during prolonged metabolic stress. It has been associated with multiple functions, including axon membrane trafficking, axonal homeostasis, mitophagy, adipose differentiation, and hematopoietic stem cell maintenance. Alternative splicing results in multiple transcript variants. |
Other Clones
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Other Formats
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Unconjugated
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