Recombinant ARG1/Arginase 1 Monoclonal Antibody (AN300403P)
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For research use only.
| Verified Samples |
Verified Samples in WB:?HepG2 Verified Samples in IHC: Human liver, Human kidney Verified Samples in IF: Hela Verified Samples in FCM: HepG2 |
| Dilution | WB 1:500-1:1000, IHC-P 1:500-1:2500, ICC/IF 1:20-1:100, FCM 1:100-1:500 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB, IHC-P, FCM, ICC/IF |
| Clonality | Monoclonal |
| Immunogen | Recombinant Human ARG1/Arginase 1 protein |
| Abbre | ARG1 |
| Synonyms | ARGI, ARG, ARG1, arginase-1, A I, Al, ARG 1, ARGI1, Arginase, Arginase 1, Arginase I, Arginase liver, Arginase type I, Arginase1, liver, Liver Arginase, Liver type arginase, Liver-type arginase, Type I arginase, A I, Al, ARG 1, arg1, ARGI1, Arginase 1, Arginase liver, Arginase type I, Arginase, liver, Arginase-1, Arginase1, Liver type arginase, Liver-type arginase, Type I arginase, Arginase |
| Swissprot | |
| Calculated MW | 35 kDa |
| Observed MW |
40 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm;Cytoplasmic granule |
| Tissue Specificity | Within the immune system initially reported to be selectively expressed in granulocytes (polymorphonuclear leukocytes [PMNs]) |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Signal Transduction, Metabolism |
| Clone No. | 9D10 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | Key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively; the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys. Functions in L-arginine homeostasis in nonhepatic tissues characterized by the competition between nitric oxide synthase (NOS) and arginase for the available intracellular substrate arginine. Arginine metabolism is a critical regulator of innate and adaptive immune responses. Involved in an antimicrobial effector pathway in polymorphonuclear granulocytes (PMN). Upon PMN cell death is liberated from the phagolysosome and depletes arginine in the microenvironment leading to suppressed T cell and natural killer (NK) cell proliferation and cytokine secretion. |
Other Clones
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