Recombinant Angiotensin II Type 1 Receptor Monoclonal Antibody (E-AB-81528)
For research use only.
| Verified Samples |
Verified Samples in WB: HepG2 |
| Dilution | WB 1:1000-1:2000 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB |
| Clonality | Rabbit Monoclonal |
| Immunogen | A synthetic peptide of human Angiotensin II Type 1 Receptor |
| Abbre | Angiotensin II Type 1 Receptor |
| Synonyms | AG2S, AGTR1, AGTR1B, AT1, AT1AR, AT1B, AT1BR, AT1R, AT2R1, HAT1R |
| Swissprot | |
| Calculated MW | 41 kDa |
| Observed MW |
41 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cell membrane. |
| Concentration | 300 μg/mL |
| Buffer | 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% stabilizer and 0.05% protective protein. |
| Purification Method | Affinity Purified |
| Research Areas | Cancer, Cardiovascular, Metabolism |
| Clone No. | R07-1I6 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | Angiotensin II (Ang II),the main effector molecule of the renin-angiotensin system,exerts its actions mainly via interaction with type-1 angiotensin II receptor (AGTR1,also named as AT1R),thereby contributing to blood pressure regulation. AGTR1 mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. By regulating vascular tone,cardiovascular function,salt and water homeostasis,AGTR1 exerts an indispensable physiological role . AGTR1 has been implicated in diverse aspects of human disease,from the regulation of blood pressure and cardiovascular homeostasis to cancer progression . |
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Other Formats
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Unconjugated
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