Recombinant Angiopoietin-2/ANG 2 Monoclonal Antibody (AN300346P)
For research use only.
| Verified Samples |
Verified Samples in WB:?HepG2, A549 Verified Samples in IF: A549 Verified Samples in IP: HepG2 |
| Dilution | WB 1:500-1:1000, ICC/IF 1:100-1:500, IP 0.2-1 μL/mg of lysate |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB, ICC/IF, IP |
| Clonality | Monoclonal |
| Immunogen | Recombinant Human Angiopoietin-2/ANG 2 protein |
| Abbre | ANGPT2 |
| Synonyms | angiopoitin, ANGP, Ang, ANGPT2, AGPT2, ANG2, AGPT 2, ANG 2, angiopoietin 2, Angiopoietin 2a, Angiopoietin 2B, Angiopoietin2, angiopoietin-2a, angiopoietin-2B, angiopoitin 2, ANGP2, ANGPT 2, Tie2 ligand, Tie2-ligand, Angiopoietin-2, ANG-2, Agpt2, ANG2, Angiopoietin 2a, Angiopoietin 2B, Angiopoietin2, ANGPT 2, Angpt2, Tie2 ligand |
| Swissprot | |
| Calculated MW | 57 kDa |
| Observed MW |
70 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Secreted |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Cardiovascular |
| Clone No. | 6A6 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal. Involved in the regulation of lymphangiogenesis. |
Other Clones
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Other Formats
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Unconjugated
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