Recombinant Amyloid-β Monoclonal Antibody (AN300886L)
For research use only.
| Verified Samples | Verified Samples in WB: SH-SY5Y |
| Dilution | WB 1:2000-1:10000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human Amyloid-β protein |
| Abbre | Amyloid-β |
| Synonyms | preA, Amyloid beta A, Beta-amyloid protein, Amyloid Precursor Protein, APP A4 AD, AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, Amyloid beta A4, APP, Aβ42, preA4, Abeta 40, amyloid beta 40, Amyloid Beta 42, Amyloid Precursor, Amyloid Precursor Protein 695, APP695, Aβ(1-40), Aβ(1-42), Aβ40, Beta-amyloid protein 40, APP A4 AD1, A4, A4 amyloid protein, AICD-50, AICD-57, AICD-59, AID(50), AID(57), AID(59), Alzheimer disease amyloid protein, Amyloid beta (A4) precursor protein, Amyloid beta A4 protein, Amyloid beta precursor protein, Amyloid beta protein, Amyloid intracellular domain 50, Amyloid intracellular domain 57, Amyloid intracellular domain 59, Beta amyloid peptide, Beta-amyloid precursor protein, Beta-APP40, Beta-APP42, C31, Cerebral vascular amyloid peptide, Gamma-CTF(50), Gamma-CTF(57), Gamma-CTF(59), peptidase nexin II, PN 2, PN II, Protease nexin II, Protease nexin-II, S-APP-alpha, S-APP-beta, Soluble APP-beta |
| Swissprot | |
| Calculated MW | 87 kDa |
| Observed MW |
100 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Membrane |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Neuroscience |
| Clone No. | 5C9 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind toThe acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others formThe protein basis ofThe amyloid plaques found inThe brains of patients with Alzheimer disease. In addition, two ofThe peptides are antimicrobial peptides, having been shown to have bacteriocidal and antifungal activities. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene. |
Other Clones
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Unconjugated
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