Recombinant ALK-1/ACVRL1 Monoclonal Antibody (AN300174P)
For research use only.
| Verified Samples |
Verified Samples in WB: MCF7, K562 Verified Samples in IP: MCF-7, K562 |
| Dilution | WB 1:500-1:2000, IP 1-4 μL/mg of lysate |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB, IP |
| Clonality | Rabbit Monoclonal |
| Immunogen | Recombinant Human ALK-1 / ACVRL1 protein |
| Abbre | ACVRL1 |
| Synonyms | SKR, ORW, Activin Receptor-Like Kinase, Serine/Threonine-Protein Kinase Receptor R, ACVRL, ACVRLK, ALK, ACVRL1, ACVRLK1, ALK-1, ALK1, HHT, HHT2, ORW2, SKR3, TSR-I, Activin Receptor-Like Kinase 1, Serine/Threonine-Protein Kinase Receptor R3, TGF-B Superfamily Receptor Type I |
| Swissprot | |
| Calculated MW | 56 kDa |
| Observed MW |
41 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cell membrane |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Cardiovascular, Signal Transduction, Metabolism |
| Clone No. | 7B6 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | This gene encodes a type I cell-surface receptor for the TGF-beta superfamily of ligands. It shares with other type I receptors a high degree of similarity in serine-threonine kinase subdomains, a glycine- and serine-rich region (called the GS domain) preceding the kinase domain, and a short C-terminal tail. The encoded protein, sometimes termed ALK1, shares similar domain structures with other closely related ALK or activin receptor-like kinase proteins that form a subfamily of receptor serine/threonine kinases. Mutations in this gene are associated with hemorrhagic telangiectasia type 2, also known as Rendu-Osler-Weber syndrome 2. |
Other Clones
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Unconjugated
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