Recombinant AIFM1 Monoclonal Antibody (AN300718L)
For research use only.
| Verified Samples | Verified Samples in WB: Jurkat |
| Dilution | WB 1:2000-1:10000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human AIFM1 protein |
| Abbre | AIFM1 |
| Synonyms | CMTX, DFNX, PDCD, AIFM, Programmed Cell Death Protein, COXPD, AIF, CMT2D, CMTX4, COWCK, COXPD6, DFNX5, NADMR, NAMSD, PDCD8, AIFM1, Apoptosis-Inducing Factor 1 Mitochondrial, Programmed Cell Death Protein 8, Apoptosis inducing factor, Apoptosis inducing factor 1, Apoptosis-inducing factor 1, axonal, axonal motor-sensory, Harlequin, mAIF, MGC111425, MGC5706, mitochondrial, mitochondrion associated, motor-sensory with deafness and mental retardation (Cowchock syndrome), Neuropathy, PDCD 8, Programmed Cell Death 8, Programmed cell death 8 (apoptosis inducing factor), Programmed cell death 8 isoform 1, Programmed cell death 8 isoform 2, Programmed cell death 8 isoform 3, Programmed cell death protein 8 mitochondrial, Programmed cell death protein 8 mitochondrial precursor, Striatal apoptosis inducing factor, with deafness and mental retardation |
| Swissprot | |
| Calculated MW | 67 kDa |
| Observed MW |
67 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Mitochondrion intermembrane space, Mitochondrion inner membrane. Cytoplasm, Nucleus, Cytoplasm, perinuclear region, Proteolytic cleavage during or just after translocation into the mitochondrial intermembrane space (IMS) results in the formation of an inner-membrane-anchored mature form (AIFmit). During apoptosis, further proteolytic processing leads to a mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis (PubMed:15775970). Colocalizes with EIF3G in the nucleus and perinuclear region (PubMed:17094969), Mitochondrion intermembrane space, Mitochondrion inner membrane, Has a stronger membrane anchorage than isoform 1, Mitochondrion, Cytoplasm, cytosol, In pro-apoptotic conditions, is released from mitochondria to cytosol in a calpain/cathepsin-dependent manner, Cytoplasm. |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Cell Biology, Epigenetics and Nuclear Signaling, Cancer, Metabolism |
| Clone No. | 12G13 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6 (COXPD6), a severe mitochondrial encephalomyopathy, as well as Cowchock syndrome, also known as X-linked recessive Charcot-Marie-Tooth disease-4 (CMTX-4), a disorder resulting in neuropathy, and axonal and motor-sensory defects with deafness and mental retardation. Alternative splicing results in multiple transcript variants. |
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