Recombinant ADAM10 Monoclonal Antibody (AN300633L)
For research use only.
| Verified Samples | Verified Samples in WB: Jurkat |
| Dilution | WB 1:2000-1:10000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human ADAM10 protein |
| Abbre | ADAM10 |
| Synonyms | CDw, ADAM, ADAM metallopeptidase domain, a disintegrin and metalloprotease domain, a disintegrin and metalloproteinase domain, disintegrin and metalloproteinase domain-containing protein, ADAM10, AD10, AD18, CD156c, CDw156, HsT18717, MADM, RAK, kuz, Kuzbanian, a disintegrin and metalloprotease domain 10, a disintegrin and metalloproteinase domain 10, ADAM metallopeptidase domain 10, CD156c antigen, disintegrin and metalloproteinase domain-containing protein 10, Kuzbanian protein homolog, Mammalian disintegrin-metalloprotease, ADAM metallopeptidase domain 10, CD156c, CDw156, HsT18717, kuz, MADM, AD 10, ADA10, ADAM 10, CD 156c, disintegrin and metalloproteinase domain containing protein 10, Drosophila, homolog of, HsT 18717, Mammalian disintegrin metalloprotease |
| Swissprot | |
| Calculated MW | 84 kDa |
| Observed MW |
84 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cell membrane, Single-pass type I membrane protein, Golgi apparatus membrane, Single-pass type I membrane protein, Cytoplasmic vesicle, clathrin-coated vesicle, Cell projection, axon, Cell projection, dendrite, Cell junction, adherens junction, Cytoplasm, Is localized in the plasma membrane but is also expressed in the Golgi apparatus and in clathrin-coated vesicles derived likely from the Golgi (PubMed:12475894). During long term depression, it is recruited to the cell membrane by DLG1 (PubMed:23676497). The immature form is mainly located near cytoplasmic fibrillar structures, while the mature form is predominantly located at zonula adherens and the cell membrane (PubMed:30463011). The localization and clustering of mature ADAM10 to zonula adherens is regulated by AFDN, TSPAN33, PLEKHA7 and PDZD11 (PubMed:30463011). |
| Tissue Specificity | Expressed in spleen, lymph node, thymus, peripheral blood leukocyte, bone marrow, cartilage, chondrocytes and fetal liver. |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Cell Biology, Signal Transduction, Cancer |
| Clone No. | 6C12 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | ADAM metallopeptidase domain 10(ADAM10) Homo sapiens Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. Alternate splicing results in multiple transcript variants encoding different proteins that may undergo similar processing. |
Other Clones
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