Recombinant 53BP2/ASPP2 Monoclonal Antibody (AN300764L)
For research use only.
| Verified Samples | Verified Samples in WB: HEK-293T |
| Dilution | WB 1:1000-5000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human 53BP2/ASPP2 protein |
| Abbre | 53BP2 |
| Synonyms | 53BP, ASPP, tumor protein p53 binding protein, P53BP, TP53BP, TP53BP2, 53BP2, ASPP2, BBP, P53BP2, PPP1R13A, tumor protein p53 binding protein 2, 53BP2, ASPP2, BBP, P53BP2, PPP1R13A, tumor protein p53 binding protein 2, Apoptosis stimulating of p53 protein 2, Apoptosis stimulating protein of p53 2, Apoptosis-stimulating of p53 protein 2, Bcl2 binding protein, Bcl2-binding protein, NY REN 51 antigen, p53 binding protein 2, p53-binding protein 2, Renal carcinoma antigen NY-REN-51, Tumor suppressor p53 binding protein 2, Tumor suppressor p53-binding protein 2, ASPP2 |
| Swissprot | |
| Observed MW |
124 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm, perinuclear region. Nucleus. Note: Predominantly found in the perinuclear region. Some small fraction is nuclear. Sequester in the cytoplasm on overexpression of DDX42. |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Cell Biology |
| Clone No. | 9D2 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | This gene encodes a member of the ASPP (apoptosis-stimulating protein of p53) family of p53 interacting proteins. The protein contains four ankyrin repeats and an SH3 domain involved in protein-protein interactions. It is localized to the perinuclear region of the cytoplasm, and regulates apoptosis and cell growth through interactions with other regulatory molecules including members of the p53 family. Multiple transcript variants encoding different isoforms have been found for this gene. |
Other Clones
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Unconjugated
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