Rat sC5b-9(Soluble Terminal Complement Complex C5b-9) ELISA Kit (E-EL-R0951)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat sC5b-9. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat sC5b-9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat sC5b-9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat sC5b-9. You can calculate the concentration of Rat sC5b-9 in the samples by comparing the OD of the samples to the standard curve.

The terminal complement complex (TCC, SC5b-9) is the result of a divergent pathway that activates the complement system by the classical, bypass or MBL pathways. The membrane attack complex (MAC), a form of TCC, is a stable complex that mediates irreversible target cell membrane damage associated with complement activation. The C5b-9 complex formed in the absence of target membranes binds to naturally occurring regulatory serum proteins, such as S proteins, to form steady state SC5b-9.The status of the terminal complement pathway in a sample is indicated by measuring the concentration of TCC. The membrane attack complex, at abnormally elevated concentrations, exhibits cytotoxicity.