Rat/Porcine E2(Estradiol) ELISA Kit

Size	Price	Qty
96T	$ 495.00
48T	$ 396.00
24T	$ 150.00
96T*5	Inquire	/
96T*10	Inquire	/

Product Summary

Sensitivity	1 pg/mL
Detection Range	1.56-100 pg/mL
Sample Volume	50 μL
Total Assay Time	2 h 30 min
Reacitivity	Rat;Porcine
Specificity	This kit recognizes Rat, Porcine E2 in samples.No significant cross-reactivity or interference between Rat, Porcine E2 and analogues was observed
Recovery	80%-120%
Sample Type	serum, plasma
Detection Method	Colorimetric method, ELISA, Competitive
Assay Type	Competitive-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	12 months

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat, Porcine E2. During the reaction, Rat, Porcine E2 in the sample or standard competes with a fixed amount of Rat, Porcine E2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat, Porcine E2. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat, Porcine E2 in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Background

Estradiol is the most potent estrogen of a group of endogenous estrogen steroids which includes estrone and estriol. In women estradiol is responsible for growth of the breast and reproductive epithelia, maturation of long bones and development of the secondary sexual characteristics. Estradiol is produced mainly by the ovaries with secondary production by the adrenal glands and conversion of steroid precursors into estrogens in fat tissue.

Research Area

Signal Transduction , Neuroscience , Developmental Biology

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Rat/Porcine E2(Estradiol) ELISA Kit (E-EL-0152)

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