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Sensitivity | 0.1 ng/mL |
Detection Range | 0.16-10 ng/mL |
Sample Volume | 100 μL |
Total Assay Time | 3 h 30 min |
Reacitivity | Rat |
Specificity | This kit recognizes Rat LEP in samples. No significant cross-reactivity or interference between Rat LEP and analogues was observed |
Recovery | 80%-120% |
Sample Type | Serum, plasma and other biological fluids |
Detection Method | Colorimetric method, ELISA, Sandwich |
Assay Type | Sandwich-ELISA |
Size | 96T / 48T / 24T / 96T*5 / 96T*10 |
Storage | 2-8℃ |
Expiration Date | 12 months |
Uniport ID | P50596 |
Research Area | Cancer, Cardiovascular, Metabolism, Neuroscience, Signal Transduction, Stem Cells |
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1 Results
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1 Results
- Effects of fermented wheat germ on the placenta of high-fat diet-induced obese maternal rats: morphology, metabolism, and nutrient transport
IF:5.100
Journal:Food & Function(2025)
DOI:10.1039/D4FO05828CReactivity:Rat
Sample Type:placenta tissue
- Efficacy of Fetal Wharton's Jelly Mesenchymal Stem Cells-Derived Small Extracellular Vesicles in Metabolic Syndrome
IF:4.800
Journal:Biomolecules(2025)
DOI:10.3390/biom15010044Reactivity:Rat
Sample Type:serum
- The Effect of the 14:10-Hour Time-Restricted Feeding (TRF) Regimen on Selected Markers of Glucose Homeostasis in Diet-Induced Prediabetic Male Sprague Dawley Rats
IF:4.800
Journal:Nutrients(2025)
DOI:10.3390/nu17020292Reactivity:Rat
Sample Type:Plasma
- The cardioprotective effects of Fruitflow? against Doxorubicin-induced toxicity in rat cardiomyoblast cells H9c2 (2?1) and high-fat diet-induced dyslipidemia and pathological alteration in cardiac tissue of Wistar Albino rats
IF:6.900
Journal:BIOMEDICINE & PHARMACOTHERAPY(2024)
DOI:10.1016/j.biopha.2024.117607Reactivity:Rat
Sample Type:heart tissue
- Anthocyanin-rich black wheat as a functional food for managing type 2 diabetes mellitus: a study on high fat diet-streptozotocin-induced diabetic rats
IF:5.100
Journal:Food & Function(2024)
DOI:10.1039/D4FO05065GReactivity:Rat
Sample Type:Serum
- Sulforaphane's role in Redefining autophagic Responses in depression associated with polycystic ovarian syndrome: Unveiling the SIRT1/AMPK/LKB1 pathway connection
IF:5.000
Journal:EUROPEAN JOURNAL OF PHARMACOLOGY(2024)
DOI:10.1016/j.ejphar.2024.176477Reactivity:Rat
Sample Type:serum
- The Influence of a High-Cholesterol Diet and Forced Training on Lipid Metabolism and Intestinal Microbiota in Male Wistar Rats
IF:4.900
Journal:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES(2024)
DOI:10.3390/ijms25105383Reactivity:Rat
Sample Type:serum
- Leptin limits hepatic lipid accumulation and inflammation via vagal activation of the JAK2-STAT3/AMPK pathway
IF:4.800
Journal:JOURNAL OF NUTRITIONAL BIOCHEMISTRY(2024)
DOI:10.1016/j.jnutbio.2024.109748Reactivity:Rat
Sample Type:Serum,cerebrospinal fluid (CSF)
- Modulation of Metabolic Pathways and Protection against Cadmium-Induced Disruptions with Taxifolin-Enriched Extract
IF:4.100
Journal:ACS Omega(2024)
DOI:10.1021/acsomega.3c08989Reactivity:Rat
Sample Type:serum
- Integrating untargeted and oxylipins-targeted metabolomics to reveal the anti-obesity and hypolipidemic mechanism of conjugated linoleic acid in high-fat diet rats
IF:3.800
Journal:Journal of Functional Foods(2024)
DOI:10.1016/j.jff.2024.106182Reactivity:Rat
Sample Type:serum
Q1:Is circulating leptin the same as leptin?
Circulating leptin refers to leptin levels, with "circulating" describing the fluctuation of leptin levels.
Q2:Why is it necessary to add a protease inhibitor in tissue sample preparation during an Elisa experiment? Will it affect the detection significantly if there is no protease inhibitor?
Tissue samples may contain endogenous or exogenous proteases during processing, leading to degradation of extracted proteins. Therefore, it's necessary to add protease inhibitors during processing to ensure the integrity of target proteins. If customers can keep samples cold and handle them quickly during processing, omitting the protease inhibitor may not have a significant effect. After preparation, samples should be tested promptly or immediately aliquoted and frozen at -20°C or -80°C.
Q3:Which variant of the spike protein does this kit detect? Do you have a kit specific to detect the spike protein for the omicron variant?
This kit is designed for the original strain of the new crown virus, and the omicron variant has not been verified. However, we have verified 26 recombinant variants of the SARS-CoV-2 spike protein through the kit. For more information, customers can refer to the kit instructions (https://file.elabscience.com/Manual/covid_19/E-EL-E605 .pdf).
Q4:What is the range of enzyme activity of your IL-2 freeze-dried powder
Currently our freeze-dried powder is a concentration unit with no information on the activity unit for the time being.
Q5:What is the principle of adding stop solution to stop color reaction in ELISA experiment?
On the one hand, the activity of HRP enzyme is destroyed and the catalytic function of HRP enzyme is lost. On the other hand, the final color changes due to a change in pH.
Q6:The absorbance of the cell supernatant is less than that of the sample diluent alone, so how to concentrate the sample?
The amount of medium can be reduced for subsequent drug administration and modeling.
Q7:My sample volume is small. Can I reduce some reagents proportionally?
No, the detection system of our kits requires strict adherence to the specified sample volume to ensure accurate detection. If the sample volume is insufficient, consider diluting appropriately, but first conduct a pre-experiment to confirm the suitable dilution factor.
Q8:May I ask which type of plate to choose when ELISA testing?
According to the different bottom, it is divided into flat bottom, U-shaped bottom, V-shaped bottom and so on. The index of refraction of the flat bottom is low, which is suitable for detection in the enzyme reader. According to the color can be divided into transparent, black, white. Transparent is commonly used for the most general enzyme-linked immunoassay.
Q9:Is the TGF-β1 ELISA Kit detecting the active form of TGF-β1 or the precursor?
The active TGF-β dimer was detected
Q10:I need to measure corticosterone and testosterone in hair samples. Are there any suggested sample extraction methods?
Hair sample preparation method: Wash hair samples with methanol by adding 5 mL of HPLC-grade methanol to each sample, rotating for 3 minutes, then decanting excess methanol and rinsing hair twice. After washing, place the hair samples on aluminum foil, dry for 3 days in a protective cap. Weigh the dried hair samples and transfer them to 2ml polypropylene tubes containing stainless steel grinding beads. Place the tubes containing hair and beads in a bead beater, grind each sample for 2 minutes to produce powder. After grinding, add 1.5 mL of methanol to the tubes containing hair powder. Rotate samples slowly for 24 hours to extract steroids. Centrifuge at 10000 g for 4 minutes, transfer 0.6 mL of methanol supernatant containing steroids to new 1.5 mL microcentrifuge tubes. Dry the samples in a protective hood for 2-3 days to evaporate the methanol. Dilute the dried extract with 0.4 mL dilution buffer from the kit for detection.