Rat Kupffer Cells Isolation and Culture Kit (P-CA-613)
For research use only.
| Product Introduction | This kit is developed specifically for extracting primary rat kupffer cells. After low-temperature digestion with mixed enzymes and density gradient separation, the cells were purified using differential adhesion method to obtain rat kupffer cells. Laboratory validation showed that 1 × 10⁶ target cells could be isolated in each experiment (1 Test). Immunofluorescence identification showed that the cell purity (CD68 positive rate) was > 90%. |
| Expiration Date | 12 months |
| Matters Need Attention |
1. The experiment requires the preparation of dissection plates, ice plate, 0.25% trypsin digestion solution, and surgical instruments (including 2 ophthalmic scissors, 2 surgical straight forceps, and 1 surgical curved forceps); tissue processing dishes (glass culture dishes, 6 cm/10 cm are acceptable), 6 cm culture dishes, and several 15 mL/50 mL centrifuge tubes. 2. Before the formal experiment, it is recommended to use 1-2 normal rats for simulated anatomy to familiarize the operation process and improve the speed of tissue separation. 3. During the entire separation process, it is recommended to place the culture dish containing the tissue on an ice plate (2-8℃) to maintain low temperature, but be careful not to freeze the tissue and liquid. 4. The culture medium contains nutrients necessary for microbial growth. Please open it in an ultra-clean workbench, divide it according to the required amount, and seal the bottle mouth with a sealing film for immediate use to avoid contamination. 5. All separation solutions in this kit may experience the precipitation of crystal particles at the bottle mouth and turbidity of the solution during storage, which is a normal phenomenon and can be used with confidence. |
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