Rat Cardiac Microvascular Endothelial Cells Isolation and Culture Kit (P-CA-620)

For research use only.
Product Introduction | This kit is developed specifically for the isolation of various primary rat cardiac microvascular endothelial cells. The tissue is digested using a two-step digestion method, and the cells are purified by density gradient centrifugation. After laboratory verification, it can isolate more than 1×10⁶ cells, and the immunofluorescence identification (CD31 positive rate) more than 90%. |
Expiration Date | 12 months |
Matters Need Attention |
1. Before the formal experiment, it is recommended to use 1-2 normal rats for simulated anatomy to familiarize the operation process and improve the speed of tissue separation. 2. During the entire anatomical sampling, it is recommended to place the culture dish containing the tissue on an ice plate (2-8℃) to maintain low temperature, but be careful not to freeze the tissue and liquid due to low temperature. 3. The culture medium contains nutrients necessary for microbial growth. Please open it in a super clean workbench, divide it according to the required amount, and seal the bottle mouth with a sealing film for immediate use to avoid contamination. 4. All separation solutions in this kit may experience the precipitation of crystal particles at the bottle mouth and turbidity of the solution during storage, which is a normal phenomenon and can be used with no problem. |
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