Rad21 Polyclonal Antibody (E-AB-93246)
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For research use only.
| Verified Samples |
Verified Samples in WB: various cell lines, C6 Verified Samples in IHC: Human colon carcinoma, Mouse stomach, Rat stomach Verified Samples in IF: U2OS, Jurkat Verified Samples in IP: Jurkat |
| Dilution | WB 1:500-1:2000, IHC 1:50-1:200, IF 1:50-1:200, IP 1:50-1:200 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, IP |
| Clonality | Polyclonal |
| Immunogen | Recombinant fusion protein of human Rad21 |
| Abbre | Rad21 |
| Synonyms | HR21, HRAD21, MCD1, NXP1, SCC1, hHR21, CDLS4 |
| Swissprot | |
| Calculated MW | 71 kDa |
| Observed MW |
130 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Condensed nuclear chromosome, cytosol, nuclear matrix, nucleoplasm, nucleus, spindle pole. |
| Concentration | 1 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol. |
| Purification Method | Affinity purification |
| Research Areas | Cell Biology, Epigenetics and Nuclear Signaling, Cancer |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells. [provided by RefSeq, Jul 2008] |
Other Clones
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Other Formats
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Unconjugated
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