Rabbit IgG Affinity Agarose (EA-IP-200)
For research use only.
| Parameter Information | 1.Application Scope Affinity purified antibody, exclusion of non-specific adsorption, and negative control of rabbit antibody agarose gel in IP, CO-IP, Ch-IP and other immunoprecipitation related experiments. 2.IgG Attributes Rabbit-derived IgG. 3.Binding substrate Agarose gel particles, average particle size 100 μ M. 4.Loading Capacity 1 mL of Sepharose 4B agarose particles, covalently conjugated to 6 mg rabbit-derived IgG. 5.Liquid storage composition PBS containing preservatives and 50% glycerol. |
| Applications | IP/COIP |
| Storage | -20℃, 12 months |
| Shipping | ice bag |
| Notes |
1. This product is limited to scientific research by professionals and cannot be used for clinical diagnosis or treatment. 2. For your safety and health, please wear laboratory clothes and disposable gloves for operation. 3. This product is in the form of gel suspension, and the content of affinity gel is 50%. Before use, gently re-suspend the gel suspension, and then use it as required. 4. If the gel fades slightly, it is normal and will not affect the experimental results. 5. It is best to prepare and use the IP-WB sample on site to avoid affecting the experimental results. 6. When a little gel is mixed with the protein loading buffer, it is normal for the WB glue to appear light pink, which does not affect the experimental results. 7. Do not dry the gel, do not sonicate the gel, and do not allow the acid treatment of gel to exceed 10 minutes. 8. The amount of gel mentioned in the method is the demonstration amount prepared in small quantities, and the specific amount should be adjusted according to the actual situation. |
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