RAB38 Polyclonal Antibody (E-AB-19172)
For research use only.
| Verified Samples |
Verified Samples in WB: NIH/3T3 Verified Samples in IHC: Human liver cancer, Human prostate cancer |
| Dilution | WB 1:500-1:2000, IHC 1:50-1:200 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Immunogen | Fusion protein of human RAB38 |
| Abbre | RAB38 |
| Synonyms | Antigen NY MEL 1, Melanoma antigen NY MEL 1, Melanoma antigen NY-MEL-1, NY MEL 1, RAB 38, RAB38, RAB38 member RAS oncogene family, Rab related GTP binding protein, Rab38, Ras related protein Rab 38, Ras-related protein Rab-38, rrGTPbp |
| Swissprot | |
| Calculated MW | 24 kDa |
| Observed MW |
Refer to figures
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cell membrane. Melanosome. |
| Concentration | 1.08 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol. |
| Purification Method | Antigen affinity purification |
| Research Areas | Signal Transduction |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | Ras-related protein Rab-38 is a protein that in humans is encoded by the RAB38 gene.On the basis of human genome sequence data, the RAB38 locus on chromosome 11 is flanked proximally by tyrosinase at 11q14-q21 and distally by EED at 11q14.2-q22.3 and MYO7A at 11q13.5.In melanocytic cells RAB38 gene expression may be regulated by MITF.May be involved in melanosomal transport and docking.Involved in the proper sorting of TYRP1. |
Other Clones
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Unconjugated
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