QuicKey Pro Rat TF (Transferrin) ELISA Kit (E-OSEL-R0010)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat TF. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Rat TF are added to the micro ELISA plate wells. Rat TF in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Rat TF.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat TF. The concentration of Rat TF in the samples is then determined by comparing the OD of the samples to the standard curve.

Transferrin(TF) is also named as Beta-1 metal-binding globulin,Siderophilin and belongs to the transferrin family. It is a bilobal iron-binding protein that plays the crucial role of binding ferric iron and keeping it in solution,thereby controlling the levels of this important metal. Serum transferrin may have a further role in stimulating cell proliferation.