QuicKey Pro Rat LBP (Lipopolysaccharide Binding Protein) ELISA Kit (E-OSEL-R0015)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat LBP. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Rat LBP are added to the micro ELISA plate wells. Rat LBP in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Rat LBP.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat LBP. The concentration of Rat LBP in the samples is then determined by comparing the OD of the samples to the standard curve.

LPS-binding protein (LBP) is a protein that binds to the lipid A moiety of bacterial lipopolysaccharides (LPS),which is a glycolipid present in the outer membrane of all Gram-negative bacteria. It is involved in the immunological response to infections of Gram-negative bacteria,together with bactericidal permeability-increasing protein (BPI). LBP also interacts with the CD14 receptor and enhances its association with LPS. LBP is associated with diseases including bacterial sepsis,and is related to cytokine release in response to bacteria in immune system.