QuicKey Pro Rat ApoH (Apolipoprotein H) ELISA Kit

Size	Price	Qty
96T	$ 520.00
48T	$ 416.00
24T	$ 150.00
96T*5	Inquire	/
96T*10	Inquire	/

Product Summary

Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.

Sensitivity	4.69 pg/mL
Detection Range	7.81-500 pg/mL
Sample Volume	50 μL
Manual Operation Time	30 min
Total Assay Time	1 h 30 min
Reacitivity	Rat
Specificity	This kit recognizes ApoH in samples.No significant cross-reactivity or interference between ApoH and analogues was observed.
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	6 months

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ApoH. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Rat ApoH are added to the micro ELISA plate wells. Rat ApoH in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Rat ApoH.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat ApoH. The concentration of Rat ApoH in the samples is then determined by comparing the OD of the samples to the standard curve.

Background

Apolipoprotein H (ApoH) is a single-chain plasma protein of about 50 kD. It is a member of the complement control superfamily of proteins. ApoH is also known as beta-2-glycoprotein 1 and activated protein C-binding protein. ApoH has been implicated in a variety of physiologic pathways, including blood coagulation, hemostasis, and the production of antiphospholipid antibodies characteristic of antiphospholipid syndrome. ApoH binds negatively charged phospholipid macromolecules and circulates as a component of lipoprotein particles and as a lipid-free serum protein. ApoH may prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells. ApoH also inhibits thrombosis by blocking the activation of Coagulation Factor XI but also shows pro-coagulant activity by inhibiting the activation of Protein C. The plasma concentrations of ApoH are strongly associated with metabolic syndrome alterations and vascular disease in type 2 diabetics and could be considered as a clinical marker of cardiovascular risk.

Gene Alias	Apoh
Gene ID	287774
Uniport ID	P26644
Protein Alias	Apoh
Research Area	Cell Biology

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QuicKey Pro Rat ApoH (Apolipoprotein H) ELISA Kit (E-OSEL-R0013)

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