QuicKey Pro Mouse SELP (P-Selectin) ELISA Kit (E-OSEL-M0019)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse SELP. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse SELP are added to the micro ELISA plate wells. Mouse SELP in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Mouse SELP.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse SELP. The concentration of Mouse SELP in the samples is then determined by comparing the OD of the samples to the standard curve.

Selp gene encodes a 140 kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. The membrane protein is a calcium-dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. Alternative splice variants may occur but are not well documented.