QuicKey Pro Mouse MMP-3 (Matrix Metalloproteinase 3) ELISA Kit

Size	Price	Qty
96T	$ 520.00
48T	$ 416.00
24T	$ 150.00
96T*5	Inquire	/
96T*10	Inquire	/

Product Summary

Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.

Sensitivity	0.38 ng/mL
Detection Range	0.63-40 ng/mL
Sample Volume	50 μL
Manual Operation Time	30 min
Total Assay Time	1 h 30 min
Reacitivity	Mouse
Specificity	This kit recognizes MMP-3 in samples.No significant cross-reactivity or interference between MMP-3 and analogues was observed.
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	6 months

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse MMP-3. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse MMP-3 are added to the micro ELISA plate wells. Mouse MMP-3 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Mouse MMP-3.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse MMP-3. The concentration of Mouse MMP-3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Background

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes; such as embryonic development; reproduction; and tissue remodeling; as well as in disease processes; such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades fibronectin; laminin; collagens III; IV; IX; and X; and cartilage proteoglycans. The enzyme is thought to be involved in wound repair; progression of atherosclerosis; and tumor initiation. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3.

Gene Alias	Mmp3
Gene ID	17392
Uniport ID	P28862
Protein Alias	Mmp3
Research Area	Cancer , Cell Biology , Cardiovascular , Signal Transduction

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QuicKey Pro Mouse MMP-3 (Matrix Metalloproteinase 3) ELISA Kit (E-OSEL-M0025)

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