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QuicKey Pro Mouse CORT(Corticosterone) ELISA Kit (E-OSEL-M0001)

  • +2
All Size Price Qty
96T $ 520.00
48T $ 416.00
24T $ 150.00
96T*5 Inquire /
96T*10 Inquire /
Add to cart

For research use only.

Product Summary

Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.

Sensitivity 4.98 ng/mL
Detection Range 12.5-800 ng/mL
Sample Volume 50 μL
Total Assay Time 1 h 30 min
Reacitivity Mouse
Specificity This kit recognizes Mouse Cort in samples.No significant cross-reactivity or interference between Mouse Cort and analogues was observed
Recovery 80%-120%
Sample Type Serum, plasma, Urine, Saliva
Detection Method Colorimetric method, ELISA, Competitive
Assay Type Competitive-ELISA
Size 96T / 48T / 24T / 96T*5 / 96T*10
Storage 2-8℃
Expiration Date 6 months
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse Cort. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse Cort are added to the micro ELISA plate wells. Mouse Cort in samples (or standards) competes with a fixed amount of Cort on the solid phase supporter for sites on the HRP linked detection antibody specific to Cort. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Mouse Cort in the samples is then determined by comparing the OD of the samples to the standard curve.
Corticosterone (CORT) administration in drinking water has been widely used by researchers. CORT is a principal GC in rodents because their adrenals do not express 17α-hydroxylase and 17, 20-lyase (CYP17A1), which are key enzymes in the synthesis of both cortisol and adrenal androgens. As CORT is hydrophobic, it must be dissolved in 100% ethanol and then diluted with water to the suitable CORT concentration with 1% ethanol content.
Research Area Cell Biology
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