QuicKey Pro Mouse CHEM (Chemerin) ELISA Kit (E-OSEL-M0018)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CHEM. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse CHEM are added to the micro ELISA plate wells. Mouse CHEM in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Mouse CHEM.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse CHEM. The concentration of Mouse CHEM in the samples is then determined by comparing the OD of the samples to the standard curve.

Chemerin, also known as Tazarotene-induced Gene 2, (Tig-2), is a member of the Cystatin superfamily. Chemerin is synthesized as a 163 aa precursor that contains a hydrophobic 20 aa N-terminal sequence, an intervening 137 aa Cystatin fold-containing domain, and a six aa C-terminal prosegment. It is activated by proteases of the coagulation, fibrinolytic, and inflammatory cascades. Chemerin is a chemoattractant for cells bearing the Chem R23 receptor, including plasmacytoid dendritic cells and tissue macrophages.