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For research use only.
Product Summary
Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.
| Sensitivity | 46.88 pg/mL |
| Detection Range | 78.13-5000 pg/mL |
| Sample Volume | 50 μL |
| Manual Operation Time | 30 min |
| Total Assay Time | 1 h 30 min |
| Reacitivity | Mouse |
| Specificity | This kit recognizes ARG1 in samples.No significant cross-reactivity or interference between ARG1 and analogues was observed. |
| Recovery | 80%-120% |
| Sample Type | Serum, plasma and other biological fluids |
| Detection Method | Colorimetric method, ELISA, Sandwich |
| Assay Type | Sandwich-ELISA |
| Size | 96T / 48T / 24T / 96T*5 / 96T*10 |
| Storage | 2-8℃ |
| Expiration Date | 6 months |
Test Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse ARG1. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse ARG1 are added to the micro ELISA plate wells. Mouse ARG1 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Mouse ARG1.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse ARG1. The concentration of Mouse ARG1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Background
Arginase 1, also known as liver arginase, is a binuclear manganese metalloenzyme. It is a key enzyme of the urea cycle that catalyses the conversion of L-arginine into L-ornithine and urea, the final cytosolic reaction of urea formation in the mammalian liver . Arginase 1 is abundantly expressed in liver, but it is also expressed in cells and tissues that lack a complete urea cycle, including lung.
| Gene Alias | Arg1 |
| Gene ID | 11846 |
| Uniport ID | Q61176 |
| Protein Alias | Arg1 |
| Research Area | Cancer, Metabolism, Signal Transduction |
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