QuicKey Pro Human TFR1 (Transferrin Receptor 1) ELISA Kit (E-OSEL-H0057)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TFR1. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human TFR1 are added to the micro ELISA plate wells. Human TFR1 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human TFR1.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TFR1. The concentration of Human TFR1 in the samples is then determined by comparing the OD of the samples to the standard curve.

CD71,also known as transferrin receptor protein 1 (TfR1),is a transmembrane glycoprotein composed of two disulfide-linked monomers,each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex which enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.