QuicKey Pro Human TFF3 (Trefoil Factor 3, Intestinal) ELISA Kit (E-OSEL-H0058)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TFF3. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human TFF3 are added to the micro ELISA plate wells. Human TFF3 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human TFF3.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TFF3. The concentration of Human TFF3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium. This gene is expressed in goblet cells of the intestines and colon. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21.