Add to cart
For research use only.
Product Summary
Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.
| Sensitivity | 0.94 ng/mL |
| Detection Range | 1.56-100 ng/mL |
| Sample Volume | 50 μL |
| Manual Operation Time | 30 min |
| Total Assay Time | 1 h 30 min |
| Reacitivity | Human |
| Specificity | This kit recognizes PRL in samples.No significant cross-reactivity or interference between PRL and analogues was observed. |
| Recovery | 80%-120% |
| Sample Type | Serum, plasma and other biological fluids |
| Detection Method | Colorimetric method, ELISA, Sandwich |
| Assay Type | Sandwich-ELISA |
| Size | 96T / 48T / 24T / 96T*5 / 96T*10 |
| Storage | 2-8℃ |
| Expiration Date | 6 months |
Test Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PRL. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human PRL are added to the micro ELISA plate wells. Human PRL in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human PRL.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PRL. The concentration of Human PRL in the samples is then determined by comparing the OD of the samples to the standard curve.
Background
PRL gene encodes the anterior pituitary hormone prolactin. This secreted hormone is a growth regulator for many tissues, including cells of the immune system. It may also play a role in cell survival by suppressing apoptosis, and it is essential for lactation. Alternative splicing results in multiple transcript variants that encode the same protein.
| Gene Alias | PRL |
| Gene ID | 5617 |
| Uniport ID | P01236 |
| Protein Alias | PRL |
| Research Area | Signal Transduction |
Other Clones
1 Results
Other Formats
1 Results
