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For research use only.
Product Summary
Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.
| Sensitivity | 0.19 ng/mL |
| Detection Range | 0.31-20 ng/mL |
| Sample Volume | 50 μL |
| Manual Operation Time | 30 min |
| Total Assay Time | 1 h 30 min |
| Reacitivity | Human |
| Specificity | This kit recognizes PR3 in samples.No significant cross-reactivity or interference between PR3 and analogues was observed. |
| Recovery | 80%-120% |
| Sample Type | Serum, plasma and other biological fluids |
| Detection Method | Colorimetric method, ELISA, Sandwich |
| Assay Type | Sandwich-ELISA |
| Size | 96T / 48T / 24T / 96T*5 / 96T*10 |
| Storage | 2-8℃ |
| Expiration Date | 6 months |
Test Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PR3. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human PR3 are added to the micro ELISA plate wells. Human PR3 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human PR3.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PR3. The concentration of Human PR3 in the samples is then determined by comparing the OD of the samples to the standard curve.
Background
PRTN3,serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro. By cleaving and activating receptor F2RL1/PAR-2, enhances endothelial cell barrier function and thus vascular integrity during neutrophil transendothelial migration. May play a role in neutrophil transendothelial migration, probably when associated with CD177.
| Gene Alias | PRTN3 |
| Gene ID | 5657 |
| Uniport ID | P24158 |
| Protein Alias | PRTN3 |
| Research Area | Signal Transduction |
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