QuicKey Pro Human OPG (Osteoprotegerin) ELISA Kit

Size	Price	Qty
96T	$ 520.00
48T	$ 416.00
24T	$ 150.00
96T*5	Inquire	/
96T*10	Inquire	/

Product Summary

Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.

Sensitivity	0.28 ng/mL
Detection Range	0.47-30 ng/mL
Sample Volume	50 μL
Manual Operation Time	30 min
Total Assay Time	1 h 30 min
Reacitivity	Human
Specificity	This kit recognizes OPG in samples.No significant cross-reactivity or interference between OPG and analogues was observed.
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	6 months

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human OPG. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human OPG are added to the micro ELISA plate wells. Human OPG in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human OPG.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human OPG. The concentration of Human OPG in the samples is then determined by comparing the OD of the samples to the standard curve.

Background

The protein encoded by TNFRSF11B gene is a member of the TNF-receptor superfamily. This protein is an osteoblast-secreted decoy receptor that functions as a negative regulator of bone resorption. This protein specifically binds to its ligand, osteoprotegerin ligand, both of which are key extracellular regulators of osteoclast development. Studies of the mouse counterpart also suggest that this protein and its ligand play a role in lymph-node organogenesis and vascular calcification. Alternatively spliced transcript variants of this gene have been reported, but their full length nature has not been determined.

Gene Alias	TNFRSF11B
Gene ID	4982
Uniport ID	O00300
Protein Alias	TNFRSF11B
Research Area	Cancer , Immunology

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QuicKey Pro Human OPG (Osteoprotegerin) ELISA Kit (E-OSEL-H0034)

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