QuicKey Pro Human LTF (Lactoferrin) ELISA Kit (E-OSEL-H0037)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human LTF. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human LTF are added to the micro ELISA plate wells. Human LTF in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human LTF.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human LTF. The concentration of Human LTF in the samples is then determined by comparing the OD of the samples to the standard curve.

LTF gene is a member of the transferrin family of genes and its protein product is found in the secondary granules of neutrophils. The protein is a major iron-binding protein in milk and body secretions with an antimicrobial activity, making it an important component of the non-specific immune system. The protein demonstrates a broad spectrum of properties, including regulation of iron homeostasis, host defense against a broad range of microbial infections, anti-inflammatory activity, regulation of cellular growth and differentiation and protection against cancer development and metastasis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.