QuicKey Pro Human LTF (Lactoferrin) ELISA Kit

Size	Price	Qty
96T	$ 520.00
48T	$ 416.00
24T	$ 150.00
96T*5	Inquire	/
96T*10	Inquire	/

Product Summary

Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.

Sensitivity	0.19 ng/mL
Detection Range	0.31-20 ng/mL
Sample Volume	50 μL
Manual Operation Time	30 min
Total Assay Time	1 h 30 min
Reacitivity	Human
Specificity	This kit recognizes LTF in samples.No significant cross-reactivity or interference between LTF and analogues was observed.
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	6 months

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human LTF. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human LTF are added to the micro ELISA plate wells. Human LTF in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human LTF.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human LTF. The concentration of Human LTF in the samples is then determined by comparing the OD of the samples to the standard curve.

Background

LTF gene is a member of the transferrin family of genes and its protein product is found in the secondary granules of neutrophils. The protein is a major iron-binding protein in milk and body secretions with an antimicrobial activity, making it an important component of the non-specific immune system. The protein demonstrates a broad spectrum of properties, including regulation of iron homeostasis, host defense against a broad range of microbial infections, anti-inflammatory activity, regulation of cellular growth and differentiation and protection against cancer development and metastasis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Gene Alias	LTF
Gene ID	4057
Uniport ID	P02788
Protein Alias	LTF
Research Area	Immunology , Signal Transduction

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QuicKey Pro Human LTF (Lactoferrin) ELISA Kit (E-OSEL-H0037)

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