QuicKey Pro Human LH (Luteinizing Hormone) ELISA Kit (E-OSEL-H0027)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human LH. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human LH are added to the micro ELISA plate wells. Human LH in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human LH.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human LH. The concentration of Human LH in the samples is then determined by comparing the OD of the samples to the standard curve.

LHB gene is a member of the glycoprotein hormone beta chain family and encodes the beta subunit of luteinizing hormone (LH). Glycoprotein hormones are heterodimers consisting of a common alpha subunit and an unique beta subunit which confers biological specificity. LH is expressed in the pituitary gland and promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids. The genes for the beta chains of chorionic gonadotropin and for luteinizing hormone are contiguous on chromosome 19q13.3. Mutations in this gene are associated with hypogonadism which is characterized by infertility and pseudohermaphroditism.