QuicKey Pro Human INS (Insulin) ELISA Kit (E-OSEL-H0019)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human INS. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human INS are added to the micro ELISA plate wells. Human INS in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human INS.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human INS. The concentration of Human INS in the samples is then determined by comparing the OD of the samples to the standard curve.

Insulin is a peptide hormone,produced by beta cells of the pancreas,and is central to regulating carbohydrate and fat metabolism in the body. It participates in glucose utilization,protein synthesis and in the formation and storage of neutral lipids. Insulin is synthesized as a precursor molecule,proinsulin,which is processed prior to secretion. A- and B-peptides are joined together by a disulfide bond to form insulin,while the central portion of the precursor molecule is cleaved and released as the C-peptide. Defects in insulin results in type 1 diabetes mellitus. Insulin may also exist 36 kDa form corresponding to the hexameric insulin form.