QuicKey Pro Human FABP1 (Fatty Acid Binding Protein 1, Liver) ELISA Kit (E-OSEL-H0030)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FABP1. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human FABP1 are added to the micro ELISA plate wells. Human FABP1 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human FABP1.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human FABP1. The concentration of Human FABP1 in the samples is then determined by comparing the OD of the samples to the standard curve.

FABP1 gene encodes the fatty acid binding protein found in liver. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. This protein and FABP6 (the ileal fatty acid binding protein) are also able to bind bile acids. It is thought that FABPs roles include fatty acid uptake, transport, and metabolism.