QuicKey Pro Human COMP (Cartilage Oligomeric Matrix Protein) ELISA Kit

Size	Price	Qty
96T	$ 520.00
48T	$ 416.00
24T	$ 150.00
96T*5	Inquire	/
96T*10	Inquire	/

Product Summary

Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.

Sensitivity	0.38 ng/mL
Detection Range	0.63-40 ng/mL
Sample Volume	50 μL
Manual Operation Time	30 min
Total Assay Time	1 h 30 min
Reacitivity	Human
Specificity	This kit recognizes COMP in samples.No significant cross-reactivity or interference between COMP and analogues was observed.
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	6 months

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human COMP. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human COMP are added to the micro ELISA plate wells. Human COMP in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human COMP.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human COMP. The concentration of Human COMP in the samples is then determined by comparing the OD of the samples to the standard curve.

Background

The protein encoded by COMP gene is a noncollagenous extracellular matrix (ECM) protein. It consists of five identical glycoprotein subunits, each with EGF-like and calcium-binding (thrombospondin-like) domains. Oligomerization results from formation of a five-stranded coiled coil and disulfides. Binding to other ECM proteins such as collagen appears to depend on divalent cations. Mutations can cause the osteochondrodysplasias pseudochondroplasia (PSACH) and multiple epiphyseal dysplasia (MED).

Gene Alias	COMP
Gene ID	1311
Uniport ID	P49747
Protein Alias	COMP
Research Area	Signal Transduction , Cell Biology

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QuicKey Pro Human COMP (Cartilage Oligomeric Matrix Protein) ELISA Kit (E-OSEL-H0056)

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