elabscienceelabscience
My Cart
Toll-free:1-888-852-8623

All categories

  • All categories
  • Flow Cytometry Antibodies
  • ELISA Kits
  • MACS Cell Isolation
  • Antibodies and Reagents
  • Apoptosis and Cell Health Detection
  • Metabolism Assays
  • Immunoassays
  • Cell Identification Kits
  • Proteins and Peptides
  • Cell Culture
Please enter the item number/product keyword!
Keyword cannot be empty !
INSERT SYMBOLS:
  • α
  • β
  • γ
  • δ
  • ε
  • ζ
  • η
  • θ
  • κ
  • μ
  • ω
  • σ
  • τ
  • λ
  • Ⅰ
  • Ⅱ
  • Ⅲ
  • Ⅳ
  • Ⅴ
  • Ⅵ
  • Ⅶ
  • Ⅷ
  • Ⅸ
  • Ⅹ
  • Ⅺ
  • Ⅻ
  • ⅩⅢ
  • ⅩⅢ
  • ⅩⅣ
  • ⅩⅤ
  • ⅩⅦ
  • ⅩⅧ
  • UP ↑
|Register My Cart 1

QuicKey Pro Human C5a (Complement Component 5a) ELISA Kit (E-OSEL-H0029)

Copy the product info.
Citations ({{ citationsPage.numTotal }}) Manual MSDS
Resources
All Size Price Qty
96T $ 520.00
48T $ 416.00
24T $ 150.00
96T*5 Inquire /
96T*10 Inquire /
Bulk request >>
Add to cart

For research use only.

Product Summary

Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.

Sensitivity 46.88 pg/mL
Detection Range 78.13-5000 pg/mL
Sample Volume 50 μL
Manual Operation Time 30 min
Total Assay Time 1 h 30 min
Reacitivity Human
Specificity This kit recognizes C5a in samples.No significant cross-reactivity or interference between C5a and analogues was observed.
Recovery 80%-120%
Sample Type Serum, plasma and other biological fluids
Detection Method Colorimetric method, ELISA, Sandwich
Assay Type Sandwich-ELISA
Size 96T / 48T / 24T / 96T*5 / 96T*10
Storage 2-8℃
Expiration Date 6 months
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human C5a. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human C5a are added to the micro ELISA plate wells. Human C5a in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human C5a.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human C5a. The concentration of Human C5a in the samples is then determined by comparing the OD of the samples to the standard curve.
C5 gene encodes a component of the complement system, a part of the innate immune system that plays an important role in inflammation, host homeostasis, and host defense against pathogens. The encoded preproprotein is proteolytically processed to generate multiple protein products, including the C5 alpha chain, C5 beta chain, C5a anaphylatoxin and C5b. The C5 protein is comprised of the C5 alpha and beta chains, which are linked by a disulfide bridge. Cleavage of the alpha chain by a convertase enzyme results in the formation of the C5a anaphylatoxin, which possesses potent spasmogenic and chemotactic activity, and the C5b macromolecular cleavage product, a subunit of the membrane attack complex (MAC). Mutations in this gene cause complement component 5 deficiency, a disease characterized by recurrent bacterial infections. Alternative splicing results in multiple transcript variants.
Gene Alias C5
Gene ID 727
Uniport ID P01031
Protein Alias C5
Research Area Immunology
Other Clones

{{antibodyDetailsPage.numTotal}} Results

Other Formats

{{formatDetailsPage.numTotal}} Results

  • IF：{{item.impact}}

    Journal：{{item.journal}} ({{item.year}})

    DOI：{{item.doi}}

    Reactivity：{{item.species}}

    Sample Type：{{item.organization}}

Species

{{relatedElisaPage.numTotal}} Results

  • Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}}

Our website uses cookies to enhance user experience. Read our Cookie Policy to find out more.