QuicKey Pro Human ALB (Albumin) ELISA Kit (E-OSEL-H0025)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ALB. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human ALB are added to the micro ELISA plate wells. Human ALB in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human ALB.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ALB. The concentration of Human ALB in the samples is then determined by comparing the OD of the samples to the standard curve.

ALB is binds water,Ca2+,Na+,K+,fatty acids,hormones,bilirubin and drugs (Probable). Its main function is the regulation of the colloidal osmotic pressure of blood (Probable). Major zinc transporter in plasma,typically binds about 80% of all plasma zinc.Major calcium and magnesium transporter in plasma,binds approximately 45% of circulating calcium and magnesium in plasma.Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner.The shared binding site between zinc and calcium at residue Asp-273 suggests a crosstalk between zinc and calcium transport in the blood (By similarity).The rank order of affinity is zinc > calcium > magnesium.Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin,and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli.Does not prevent iron uptake by the bacterial siderophore aerobactin.