Pyruvate Decarboxylase (PDC) Activity Assay Kit (E-BC-K500-M)
For research use only.
| Detection Principle | Pyruvate decarboxylase (PDC) mainly exists in yeast and is one of the key enzymes in ethanol fermentation. PDC catalyzes pyruvate decarboxylate to acetaldehyde, which reacts under the action of ethanol dehydrogenase (ADH), and catalyzes NADH to convert into NAD+. NADH has a characteristic absorption peak at 340 nm. The PDC activity can be calculated by measuring the OD value at 340 nm. |
| Synonyms | PDC |
| Sample Type | Serum,Plasma,Tissue,Cell sample |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (330-350 nm, optimum wavelength: 340 nm) |
| Research Area | Glycolysis And Lipid Metabolism |
| Other Reagents Required | Normal saline (0.9% NaCl) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.67 U/L |
| Detection Range | 0.67-27.73 U/L |
| Precision | inter-assay CV: 3.1% | intra-assay CV: 2.8% |
| Sample Volume | 20 μL |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse liver tissue homogenate | 25-50 |
| 10% Mouse kidney tissue homogenate | 25-50 |
| 10% Mouse heart tissue homogenate | 25-50 |
| Rat serum | 1 |
| Rabbit plasma | 1 |
| HL-60 cell | 3-5 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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