Protein Tyrosine Phosphatase-1B (PTP-1B) Activity Colorimetric Assay Kit (E-BC-K823-M)
For research use only.
| Detection Principle |
Protein Tyrosine Phosphatase-1B (PTP-1B) belongs to the family of protein tyrosine phosphatases, which specifically hydrolyze aromatic phosphates and negatively regulate insulin signal transduction by dephosphorylating tyrosine residues on insulin receptors or their substrates. In the dephosphorylation of PTP-1B, the phosphate group produced reacts with malachite green to form chromogenic substance, PTP-1B in samples is measured by measuring the absorbance change at 636 nm. |
| Sample Type | Animal and plant tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (630-640 nm, optimum wavelength: 636 nm) |
| Research Area | Glycolysis And Lipid Metabolism , Metabolic Diseases |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.216 U/L |
| Detection Range | 0.216-7.200 U/L |
| Precision | inter-assay CV: 7.5-10.0% | intra-assay CV: 2.0-2.1% |
| Sample Volume | 40 μL(tissue/cell homogenate) |
| Assay Time | 1 h 50 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse liver tissue homogenate | 1 |
| 10% Mouse kidney tissue homogenate | 1 |
| 10% Rat lung tissue homogenate | 1 |
| 10% Mouse brain tissue homogenate | 1 |
| 10% Green radish tissue homogenate | 1 |
| 1×10^6 Jurkat cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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