Protein Carbonyl Colorimetric Assay Kit (Tissue and Serum Samples) (E-BC-K117-M)

Copy the product info.

ManualMSDS

Resources

All	Size	Price	Qty
	96T	$ 280.00
	48T	$ 0.01

Bulk request >>

Add to cart

For research use only. Order now, ship in 3 days

Detection Principle	The content of protein carbonyl increased after oxidation, and the carbonyl group reacted with 2, 4-dinitrophenylhydrazine to form a reddish brown precipitate. The absorbance can be measured at 370 nm after the precipitation is dissolved. The carbonyl content can be calculated indirectly.
Sample Type	Serum,Plasma,Hydrothorax,Cell culture supernatant,Tissue
Detection Method	Colorimetric method
Detection Instrument	Microplate reader (360-385 nm,optimum wavelength: 370 nm)
Research Area	Oxidative Stress, Amino Acids And Proteins
Other Reagents Required	Absolute ethanol, Ethyl acetate
Storage	This product can be stored at 2-8°C for 12 months with shading light.
Valid Period	12 months
Precision	inter-assay CV: 8.5% \| intra-assay CV: 5.2%
Sample Volume	0.2 mL
Assay Time	2 h 20 min

Cat.No.	Product Name	Sizes
E-BC-K171-M	Total Carbonyl Colorimetric Assay Kit	96T , 48T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
Human serum	8-10
Mouse serum	8-10
10% Rat liver tissue homogenate	2-3
10% Mouse brain tissue homogenate	1
Human milk	1
Human urine	1
10% Mouse heart tissue homogenate	1
10% fish tissue homogenate	1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.