Primary Cell Cryopreservation Solution（SFC1） (PB180439)
For research use only.
| Product Introduction |
In vitro cell culture, in order to preserve the biological activity of cells for a long time, the cells must be cryopreserved,and resuscitated when necessary.At present, the most commonly used method for cell cryopreservation is liquid nitrogen method, which mainly uses the addition of an appropriate amount of protective agent to slowly cool the cells to a specified temperature range, so as to achieve the purpose of protecting cells. The Primary Cell Cryopreservation Solution (SFC1) Medium is a special cryopreservation product developed by Pricella for primary cells, mainly for the cryopreservation of mesenchymal stem cells, smooth muscle cells, fibroblasts and chondrocytes derived from a variety of tissue species. The product formula contains a self-developed cryoprotectant and a dual cryoprotectant of 10% DMSO. The product formula is clear , does not contain serum or animal-derived proteins, which can reduce contamination risk from bacteria, viruses, and mycoplasma, ensuring the safety of frozen cells. Compared with traditional cryopreservation media, there is no need for cumbersome and time-consuming procedural cryopreservation steps or expensive procedural coolers, and the cells can be directly resuspended and placed in an ultra-low temperature freezer at -80℃ to ensure that the ultra-low temperature temperature is stable and frozen for 1 year. If you want to store liquid nitrogen for a medium and long term, you need to put it in a -80°C ultra-low temperature freezer for 24 h before moving it to a liquid nitrogen tank for long-term storage. |
| Format | Liquid |
| pH | 7.4-7.6 |
| Animal Origin Ingredient | Without |
| Green Features | Sustainable packaging |
| Storage | This product can be stored at 2-8℃ for 12 months. |
| Shipping | Ice bag |
| Expiration Date | 12 months |
| Matters Need Attention |
1. After the cryopreserved cells are aliquoted into cryopreservation tubes, the storage time at room temperature/4 °C should be minimized, and they should be moved to the -80 °C ultra-low temperature freezer as soon as possible; 2. When using this product to cryopreserve primary cells, please ensure that the cryopreserved cells are in the previous generation, the morphology is good, the cell viability is >90%, and the number of cryosurviable cells is 2-5×106/mL/tube, and the amount of cells in each tube is 0.5-1 mL; 3. It is suitable for cryopreservation of a variety of primary cells, some tissue species derived from primary cells may have cell recovery viability of less than 90% due to the sensitivity of cell cryopreservation, it is recommended that you conduct at least 1 week of cell cryopreservation test experiment on the cells to be frozen before use, and then carry out formal cryopreservation after confirming that there is no problem; 4. At present, the product has been verified to be suitable for cells, including: mesenchymal stem cells from a variety of tissue species, smooth muscle cells, and fibroblasts cells and chondrocytes; If you are cryopserving these types of cells for the first time, it is recommended that you perform at least 1 week of cell cryopreservation test experiments on the cells to be frozen before use, and confirm that there is no problem before formal cryopreservation; 5. This product has been filtered and sterilized by 0.22 μm for 3 times, and aseptic operation should be paid attention to when using this product to avoid contamination; 6. For your safety and health, please wear a lab coat and disposable gloves to operate; 7. This product is only for scientific research purposes and should not be used for diagnostic, therapeutic, clinical and other purposes. |
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