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Porcine LDLR (Low Density Lipoprotein Receptor) ELISA Kit (E-EL-P3015)

All Size Price Qty
96T $ 682.00
48T $ 546.00
24T $ 150.00
96T*5 Inquire /
96T*10 Inquire /
Add to cart

For research use only.

Product Summary
Sensitivity 4.69 ng/mL
Detection Range 7.81-500 ng/mL
Sample Volume 100 μL
Total Assay Time 3 h 30 min
Reacitivity Porcine
Specificity This kit recognizes LDL R in samples.No significant cross-reactivity or interference between LDL R and analogues was observed.
Recovery 80%-120%
Sample Type Serum, plasma and other biological fluids
Detection Method Colorimetric method, ELISA, Sandwich
Assay Type Sandwich-ELISA
Size 96T / 48T / 24T / 96T*5 / 96T*10
Storage 2-8℃
Expiration Date 12 months
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to LDLR . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for LDLR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain LDLR , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of LDLR . You can calculate the concentration of LDLR in the samples by comparing the OD of the samples to the standard curve.
LDLR (low density lipoprotein receptor) is a member of the LDL receptor gene family and is involved in receptor-mediated endocytosis of specific ligands. The LDLR is a cell surface glycoprotein that scavenges LDL from the blood and regulates plasma LDL cholesterol. The cytoplasmic domain of the LDL receptor is necessary for the receptor to cluster in coated pits, which promotes the rapid endocytosis of bound LDL. The protein is highly glycosylated through N- and O-linkages and thus migrates at 100 to 160 kDa bands on SDS-PAGE.
Uniport ID Q28832
Research Area Cell Biology , Neuroscience , Synaptic Biology
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