For research use only.
| Verified Samples | Verified Samples in WB: HepG2, A549, SH-SY5Y, Mouse muscle, Mouse brain, Rat muscle, Rat brain |
| Dilution | WB 1:500-1:2000 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| Clonality | Polyclonal |
| Immunogen | Recombinant protein corresponding to Mouse PKM2 |
| Abbre | PKM |
| Synonyms | CTHBP, Cytosolic thyroid hormone binding protein, Cytosolic thyroid hormone-binding protein, KPYM, MGC3932, OIP 3, OIP-3, OIP3, OPA interacting protein 3, Opa-interacting protein 3, PK, PK muscle type, PK2, PK3, PKM, PKM2, Pyruvat, Pyruvate kinase 2/3, muscle type, p58, pykm |
| Swissprot | |
| Calculated MW | 58 kDa |
| Observed MW | 58 kDa Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm. Nucleus. Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic actvity. |
| Concentration | 440 μg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 1% protein protectant and 50% glycerol. |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Epigenetics and Nuclear Signaling, Metabolism, Signal Transduction |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | PKM2,also named as OIP3,PK2,PK3,PKM,p58,THBP1,CTHBP and Tumor M2-PK,belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP,generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM2 plays a general role in caspase independent cell death of tumor cells. The activity of the M2 isoform (but not the M1 isoform) can be inhibited by tyrosine kinase signalling in tumourcells.The primary pyruvate kinase isoform before tumour development is PK-M1; however,the primary isoform from four independent tumours is PK-M2. PKM2,Pyruvate kinase isozymes M1/M2,has 2 isoforms .The immunogen of this antibody is M2 isoform,also called PKM2. |
Other Clones
1 Results
Other Formats
1 Results
Unconjugated
