PI 3 kinase p85 alpha Monoclonal Antibody (E-AB-22164)
For research use only.
| Verified Samples |
Verified Samples in WB: 3T3, Rat liver Verified Samples in IHC: Rat liver, Mouse brain |
| Dilution | WB 1:1000-2000, IHC 1:100-200 |
| Isotype | IgG |
| Host | Mouse |
| Reactivity | Mouse, Rat |
| Applications | WB, IHC-p |
| Clonality | Monoclonal |
| Immunogen | Recombinant Protein |
| Abbre | PI3 Kinase P85α |
| Synonyms | GRB1, P85A, Phosphatidylinositol 3 kinase, Phosphatidylinositol 3 kinase associated p 85 alpha, Phosphatidylinositol 3 kinase regulatory 1, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, p85, p85 alpha, polypeptide 1 (p85 alpha), regulatory subunit |
| Swissprot | |
| Observed MW |
85 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytosol, Endoplasmic reticulum, perinuclear endoplasmic reticulum membrane, Nucleus, Plasma Membrane, Other locations: cell-cell junction, cis-Golgi network, cytoplasm, membrane, phosphatidylinositol 3-kinase complex, phosphatidylinositol 3-kinase complex, class IA, protein complex. |
| Tissue Specificity | Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level). |
| Concentration | 1 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 0.5% protein protectant and 50% glycerol. |
| Purification Method | Protein A purification |
| Research Areas | Cancer, Metabolism, Immunology, Signal Transduction |
| Clone No. | 3B7 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. |
Other Clones
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Other Formats
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Unconjugated
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