Phospholipase D (PLD) Activity Colorimetric Assay Kit (E-BC-K783-M)
For research use only.
| Detection Principle |
Phospholipase D (PLD) exists widely in natural plants and animals, and PLD plays a key role in phospholipase metabolism. PLD hydrolyzes phosphodiester bonds of glycerol phospholipids to produce phosphatidylic acid and nitrogenous base. Abnormal PLD activity and expression are associated with Alzheimer's disease, stroke, cancer, and other brain diseases. The detection principle of this kit is that PLD catalyze the substrate and the chromogenic agent to product colored substance, which has the maximum absorption at the wavelength of 550 nm. By measuring the OD value at 550 nm, PLD activity in the sample is calculated according to the standard curve. |
| Sample Type | Serum,Plasma,Animal and plant tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (540-560 nm, optimum wavelength: 550 nm) |
| Research Area | Glycolysis And Lipid Metabolism |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.68 U/L |
| Detection Range | 0.68-16.73 U/L |
| Precision | inter-assay CV: 1.8-9.5% | intra-assay CV: 1.7-2.8% |
| Sample Volume | 20 μL |
| Assay Time | 50 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Mouse serum | 1 |
| Mouse plasma | 1 |
| Human serum | 1 |
| Rabbit plasma | 1 |
| 10% Mouse liver tissue homogenate | 2-4 |
| 10% Mouse kidney tissue homogenate | 2-4 |
| 10% Mouse heart tissue homogenate | 2-4 |
| 10% Rat liver tissue homogenate | 2-4 |
| 10% Peanut tissue homogenate | 1-3 |
| 10% Edamame tissue homogenate | 1-3 |
| 10% Corn seed tissue homogenate | 1-3 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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